Just upload files you want to join together, reorder them with draganddrop if you need and click join files button to merge the documents. The longterm persistence and activity of a naked plasmid dna pgl3 35s containing a luc gene reporter gene controlled by a plant 35s camv promoter was studied in atlantic salmon salmo salar l. This document contains steps on how to make the browser download pdf files automatically, rather than open in a new tab. H4iie rat hepatoma cells were cultured as previously described 8,15. This is a free resource for the scientific community that is compiled by addgene. For the mutagenesis, the sequences complementary to the seed of the mir20a mirna in each 3 utr gcacttt were replaced by the sequence cgtgaaa.
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. You must save the document to apply the new security settings. We therefore checked the native situation in arabidopsis leaves and found a single specific band corresponding to processed pgl3 supplemental figure 7. Learn about the latest plasmid technologies and research tools. Control vector with the sv40 enhancer and promoter driving strong luciferase expression. Download free adobe acrobat reader dc software for your windows, mac os and android devices to view, print, and comment on pdf documents. A schematic diagram of a pgl4 vector is shown in figure 1. Text control reporting combines the power of a reporting tool and an easytouse wysiwyg word processor fully programmable and embeddable in. Open the pdf, then select tools protect more options remove security. Dualtargeting of arabidopsis 6phosphogluconolactonase 3. My document is too long or too large the file size limits for our online compare tool are 300 pages and 10 mb. Learn how to edit pdf files using adobe acrobat dc. After 72 h, mice were anaesthetized and given 3 mg luciferin intraperitoneally 15 min before imaging.
In the password security settings dialog box, you can add two types of passwords. Shop online for a wide selection of promega pgl3 luciferase reporter vectors four firefly luciferase reporter vectors. A series of reporter gene plasmids containing various portions of the 2069 bp mlaa34 pro. The pgl3 luciferase reporter vectors a provide a basis for the quantitative analysis of factors that potentially regulate. Reduced risk of anomalous expression the pgl3 vector backbone was used as a template for the pgl4 vector backbone. This plasmid is useful in monitoring transfection efficiency in general and is a convenient internal standard for promoter and enhancer activities expressed by pgl2 recombinants.
Document version publishers pdf, also known as version of record publication date. Original article mir19a overexpression contributes to. Soda pdf pdf software to create, convert, edit and sign files. We used a pgl3 control luciferase vector, which contains sv40 enhancer and promoter regions, to determine the effect of our treatments on the parent backbone promega. Protect your pdf file and restrict others from editing. This service enables you to rotate pdf documents permanently, free of charge. Plasmids to generate the luciferase fusion to the human 3utrs, we subcloned the 3utr fragments downstream of firefly luciferase luc in pgl3 control promega. Wait for the conversion process to finish and download files either one by one, using thumbnails, or in a zip archive. You can remove security from an open pdf if you have the permissions to do so. Pdf joiner allows you to merge multiple pdf documents and images into a single pdf file, free of charge. By default, most downloaded pdf files open in the internet browser currently in use, usually in a new window or tab.
The pgl3basic vector lacks eukaryotic promoter and enhancer sequences, allowing maximum. Welcome to vector database vector database is a digital collection of vector backbones assembled from publications and commercially available sources. Modify the content and customize the structure of your documents to better suit your needs. This plasmid is useful in monitoring transfection efficiency, in general, and is a convenient internal standard for promoter and enhancer activities expressed by pgl3 recombinants.
Rearrange individual pages or entire files in the desired order. Change text and images quickly and easily in pdf documents. The pgl2 luciferase reporter vectors are designed for the quantitative analysis of factors that potentially regulate mammalian gene expression. Click the upload files button and select up to 20 html files or zip archives containing html, images and stylesheets. Promega pgl3 luciferase reporter vectors pgl3 control. Atlantic salmon mean weight 70 grams were injected intramuscularly with 100. Running your document through optical character recognition ocr software will generate a text layer that draftable can read. See the example screenshot below showing the email notification received by a. View the article pdf and any associated supplements and figures for a period of 48 hours. We place great importance on the safe handling of your pdf documents. Numerous reports document the transcription factors and experimental treatments that transactivate or repress promoterless reporter vectors and internal control.
When you save the document, the signature and text become part of the pdf. All of these documents are available on our web site at. A functional snp in the promoter region of tcof1 is associated with reduced gene expression and yy1 dnaprotein interaction cibele masotti a, lucia m. Compare two versions of a pdf file in adobe acrobat.
The control vector contains the sv40 promoter and enhancer region and can be. Follow these steps to use the compare documents feature of adobe acrobat to compare two versions of a pdf file and get a detailed report with the differences. Joshua mendells lab contains the insert pten 3 utr wild type and is published in nature. Promega pgl3 luciferase reporter vectors pgl3 control vector life sciences. Original article mir19a overexpression contributes to heart failure through targeting adrb1 ye miao 1, hui chen2, min li. They can be signed electronically, and you can easily view pdf files on windows or mac os using the free acrobat reader dc software. Inside reader, you can subscribe to activate additional capabilities to create pdf files and export them to word or excel.
Department of pathology kyk chan, ms xu, jcy ching, yc ip, us khoo department of surgery vs chan, p tam, cl lin department of psychiatry sham p school of public health gm leung department of microbiology jsm peiris. Original article reduced mir29b3p expression upregulate. Both constructs also contain the neo gene see neo cassette, isolated from pires1neo clontech. How to change browser download settings for pdf files.
Clicking the link or the button opens the document in a browser. Only with adobe acrobat reader you can view, sign, collect and track feedback, and share pdfs for free. Recipients get an email notifying them that the file is shared with them. The pgl3 luciferase reporter vectors ac provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression.
The document open password restricts who may open the document. And when you want to do more, subscribe to acrobat pro dc. The pgl2control vector contains the sv40 promoter and enhancer sequences, resulting in strong luc expression in many types of mammalian cells. Pgl3 proved intactness but suggested inefficient transitpeptide processing of the two chimeric fusions.
To proceed, select your browser from the list below and follow the instructions. If the pdf is secured with a serverbased security policy, only the policy author or a server administrator can change it. All you need to do is select the pdf document you want to rotate on your computer, select the rotation angle and click a button. A functional snp in the promoter region of 1 is associated. The pdf is now an open standard, maintained by the international organization for standardization iso. The pgl3 control vector contains cdna encoding firefly luciferase, with expression driven by the sv40 promoter. These factors may be cisacting, such as promoters and enhancers, or transacting, such as various dnabinding factors.
A plant 35s camv promoter induces longterm expression of. Numbers correspond to the col17a1 mrna sequence roman and the pgl3 sequence italic. You can also add text, such as your name, company, title, or the date. Pdf documents can contain links and buttons, form fields, audio, video, and business logic. Sequences from the 3utr of bcl11b were cloned into the pgl3 control vector promega between the luciferase cdna and polya at the xbai restriction site fig. If you need to send a pdf to yourself or someone else but its too large to attach to an email, you can reduce pdf file size for free using our online pdf compressor without sacrificing the quality of your pdf files. For the promoter analysis, a fragment of 1 kb from themir1792cluster promoter containing four e2fbinding sites was amplified from genomic.
The permissions password restricts printing, editing, and copying based on your selections. To sign a pdf document or form, you can type, draw, or insert an image of your handwritten signature. To apply 256bit aes encryption to documents created in acrobat 8 and 9, select acrobat. Receive the latest news, hot plasmids, discounts and more. Then you can edit, export, and send pdfs for signatures. Regulation of expression of mlaa34 gene through transcriptional. Im trying clone a long insert into vector pgl3 researchgate. For more information, see b05 control vector with the sv40 enhancer and promoter driving strong. The email contains the open button and a link to the shared document. This is a free resource for the scientific community that is compiled by addgene this page is informational only this vector is not available from addgene please contact the manufacturer for further details. Recipients can also download the document if necessary. University of groningen posttranscriptional mechanisms in. Research fund for the control of infectious diseases li ka shing faculty of medicine, the university of hong kong. Original article reduced mir29b3p expression upregulate cdk6 and contributes to iga nephropathy lina xing, hao wang, peihao yin, yujun liu, yangfeng chi, yunman wang, wen peng.
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